USE OF GLYCEROL AS CRYOPROTECTANTS IN FREEZING SENTUL CHICKEN SEMEN

Junaedi Junaedi, Raden Iis Arifiantini, Cece Sumantri, Asep Gunawan

Abstract


On the freezing semen chicken cryoprotectants required to overcome the damage of spermatozoa due to cold shock. This study aims to get the best concentrations of cryoprotectants glycerol concentration of 5%, 7% and 9% in freezing Sentul chicken semen. The semen used in this study came from three chickens Sentul and be repeated nine times. Semen was collected by  messase methods for three times a week. Semen was evaluated macroscopic and microscopic. Furthermore spermatozoa diluted with egg yolk and the addition of three concentrations of cryoprotectants glycerol (5%, 7% and 9%). Semen diluted 0:25 ml is packed into straw. Then equilibrated at a temperature of 5°C for two hours. After equilibration to evaluate the motility and viability of spermatozoa. Furthermore, frozen in liquid nitrogen vapor for 10 minutes. Frozen semen is then stored in liquid nitrogen containers with temperature -196°C. After 24 hours, semen is thawed at 37°C for 30 seconds. The results showed that the percentage of sperm motility and viability of frozen semen cock Sentul using glycerol cryoprotectants 5% better P (<0.05) compared with the use of glycerol 7% and 9%. The use of glycerol 5% at this stage of equilibration and storage can reduce the damage of spermatozoa in the semen of chicken Sentul. Neither glycerol 5% could increase recovery rate after thawing

Keywords


frozen semen, chicken Sentul, glycerol

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References


Arifiantini, I. & I. Supriatna (2007). Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender. JITV, 12(2), 139-146.

Alvarenga, M.A., F.O. Papa, F.C. Landim & A.S.L. Medeiros. (2005). Amides as cryoprotectants for freezing stallion semen. Anim. Reprod. Sci., 89, 105 – 113.

Azizah & Arifiantini I, 2009. Kualitas semen beku kuda pada pengencer susu skim dengan konsentrasi gliserol yang berbeda. Jurnal Veteriner, 10 (2):,63-70.

Castillo, A.I. Romboli & M. Marzoni. (2010). Preliminary investigation on fertility and hatchability by the use of cryopreserved cock semen. Av. Biol. Res., 3, 127-128.

Dumpala, P.R., M. Parker & C.D.l. Danie. (2006). The effect of semen storage temperature and diluent type on the sperm quality index of broiler breeder semen. Int. J. Poult. Sci., 5, 838 – 845.

Ervandi, M. (2011). Pengaruh Bangsa Ayam Terhadap Kualitas Dan Fertilitas Spermatozoa. Palu: Program Studi Produksi Ternak, Jurusan Peternakan Fakultas Pertanian, Universitas Tadulako.

Fulton, J.E. (2006). Avian genetic stock preservation: An industry perspective. Poult. Sci. 85, 227 – 231

Ihsan, M.N. (2013). Pembekuan vitrivikasi semen Kambing Boer dengan tingkat gliserol berbeda. Jurnal Ternak Tropika, 14 (2), 38-45.

Iskandar, S. 2007. Tata Laksana Pemeliharaan Ayam Lokal. Bogor: Balai Penelitian Ternak Ciawi..

Junaedi, I. Arifiantini, C. Sumantri & A. Gunawan. (2016). The use of dimethyl sulfoxide as cryoprotective agent for native chiken frozen semen. Jurnal Veteriner, 17(2), 1-9.

Junaedi. (2015). Daya Tahan Pembekuan Semen Empat Genetik Ayam Lokal pada Program Kriopreservasi Plasma Nutfah Indonesia Tesis. Bogor (ID): Institut Pertanian Bogor.

Saleh, D.M. 2004. Optimization of semen processing and cryopreservation technique in Philipphine Native Roosters (Gallus gallus domesticus L). Disertasion. Los Banos: Institute of Animal Science. University of the Philipphine Los Banos.

Sartika, T. (2010). Pembentukan Galur Ayam Merawang Petelur Tahan Flu Burung (60%), Umur Bertelur 6 Bulan Dan Rataan Produksi Telur Tinggi (180 Butir/Tahun). Balai Penelitian Ternak. Pusat Penelitian dan Pengembangan Peternakan Badan Penelitian dan Pengembangan Pertanian Kementerian Pertanian.

Solihati, N.R., R. Idi, Setiawan, & Asmara. (2006). Pengaruh Lama Penyimpanan Semen Cair Ayam Buras pada Suhu 5oC terhadap Periode Fertil dan Fertilisasi Sperma. J Anim Prod., 10 (1), 22-29.

Souhoka, D.F., M.J. Matatula, W.M. Mesang-Nalley, & M. Rizal. (2009). Laktosa mempertahankan daya hidup spermatozoa kambing peranakan etawa yang dipreservasi dengan plasma semen domba priangan. J. Veteriner, 10 (3),135-142.

Suidzinska A. & E. Lukaszewicz. (2008). The effect of breed on freezability of semen of fancy fowl. Anim. Sci. Pap. Rep. 26, 331 – 340.

Yulnawati & M.A. Setiadi. (2005). Motilitas dan keutuhan membran plasma spermatozoa epididimis kucing selama penyimpanan pada suhu 4°C. J Med. Vet., 21(3), 100-104.


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